Bacterial Genetics and Physiology
GMS 6038
Fall 2011
9:35-10:25 a.m.; R2-265
Paul A. Gulig, Ph.D.
gulig@ufl.edu
Bacterial Genetics and Physiology
GMS 6038
Fall 2011
9:35-10:25 a.m.; R2-265
Paul
A. Gulig, Ph.D.
gulig@ufl.edu
Note: This course is being significantly revised for 2011. It is designed to follow GMS 6121, Infectious Diseases, which was taught in the previous spring. In that course two weeks of basic bacteriology (structure, physiology, genetics) were taught and will only be briefly reviewed in GMS 6038. If you have not had basic bacteriology before, you should check out this PowerPoint/PDF to make sure that you will be ready for the course. Here is a prinatable black and white version of the PowerPoint/PDF that won't eat ink and toner. Here is a file that has questions to let you know if you are adequately prepared from your past education and experience.
Text: Molecular Genetics of Bacteria, Third Edition, Snyder and Champness, ASM Press (now available in the Health Science Center Bookstore). THIS IS A REQUIRED TEXT.
Online Escherichia coli and Salmonella typhimurium (updated link - connection via UF network required, the print version table of contents leads to better links)
Send me an introductory email telling me your program, your research goals, how much bacteriology you've had, and anything that you'd like to learn in this course.
| Dates | Subject | Handouts | Text Reading | EcoSal Online | Other |
| Aug. 22, 24 | Introduction - Refresher on bacterial structure and physiology, antibiotics and resistance | Introduction | Outer Membrane; Peptidoglycan; Cytoplasmic Membrane; Flagella and Motility | Growth rate Outer membrane blog |
|
| Aug. 26 | Antibiotics and resistance |
Beta-lactams and vancomycin, Antibiotic resistance flow chart |
|||
| Aug. 29 | Expression: Transcription | Chapter 2 | Transcription; Translation | ||
| Aug. 31-Sept. 2 | Protein expression | X | Chapters 2, 14 | Sec-dependent; Across outer membrane | Type VI review |
| Sept. 5 | Labor Day | ||||
| Sept. 7 | Protein secretion | ||||
| Sept. 9 | General Genetics | X | (start page 10 of handout) | ||
| Sept. 12 | DNA replication | Chapter 1 | Initiation of chromosome replication; Replication fork | ||
| Sept. 14-16 | Plasmid genetics and biology | Chapter 4 | Plasmid replication | Study Guide, pCVD442 | |
| Sept. 19 | Bacteriophages | Chapters 7 and 8 | Lambda Guide, Phage display, PPT |
||
| Sept. 21 |
Paradigms of transcriptional
regulation |
Chapter 12 | |||
| Sept. 21 | Global regulation | Chapter 13 | |||
| Sept. 23 | Quorum sensing/biofilms? | X | Biofilm/Quorum Sensing study guide | Annual Review Article | |
| October 7 - 1pm - 3pm |
Final Exam | College of Medicine Testing Center (CG-23) Map | |||
ASSIGNMENTS
Homework on Growth Rate-
key
Homework
on Protein Export and Secretion -
key
Homework on Plasmids -
Key
Homework
on Regulation of Expression -
key
Old Exams
(may not necessarily match style for this year)
2011 - Exam -
Key
2010 - Exam -
Key
2009 - Exam - Key
Expression-secretion
DNA replication
Phage
Phage Display
scFv Phage Display
Regulation 1
Regulation 2
