Bacterial Genetics and Physiology
GMS 6038
Fall 2009
9:35-10:25 a.m.; R2-265
Paul A. Gulig, Ph.D.
gulig@ufl.edu
Bacterial Genetics and Physiology
GMS 6038
Fall 2009
9:35-10:25 a.m.; R2-265
Paul
A. Gulig, Ph.D.
gulig@ufl.edu
Text: Molecular Genetics of Bacteria, Third Edition, Snyder and Champness, ASM Press (now available in the Health Science Center Bookstore)
Online Escherichia coli and Salmonella typhimurium (updated link - connection via UF network required, the print version table of contents leads to better links)
**Link to Doodle to
schedule final exam.**
| Dates | Subject | Handouts | Text Reading | EcoSal Online | Other |
| Aug. 24 - Aug. 28 |
Introduction - Refresher on bacterial structure and physiology, antibiotics and resistance | Introduction | Outer Membrane; Peptidoglycan; Cytoplasmic Membrane; Flagella and Motility | Growth rate |
|
| Aug. 31, Sept. 2 | Antibiotics and resistance |
" |
Beta-lactams and vancomycin, Antibiotic resistance flow chart |
||
| Sept. 4 | Expression: Transcription and translation | Chapter 2 | Transcription; Translation | ||
| Sept. 7 | Labor Day | ||||
| Sept. 9, 11 | Protein secretion | Chapters 2, 14 | Sec-dependent; Across outer membrane | Type VI review | |
| Sept. 14 | DNA replication | Chapter 1 | Initiation of chromosome replication; Replication fork | ||
| Sept. 16, 18 | Plasmid genetics and biology | Chapter 4 | Plasmid replication | Study Guide, pCVD442 | |
| Sept. 21 | Bacteriophages | Chapters 7 and 8 | Lambda Guide, Phage display |
||
| Sept. 23 | Paradigms of transcriptional
regulation |
Chapter 12 | |||
| Sept. 25 | Global regulation | Chapter 13 | |||
| Sept. 28 | Special topic | Review article on CsrA regulation (Study questions for class discussion) | |||
| Oct. 6 (12:00-4:00) | Final Exam | R3-265 | |||
ASSIGNMENTS
Homework on Growth Rate;
Key
-
due August 31 before class
Homework
on Protein Export and Secretion; Key
-
due Sept. 11 before class
Homework on
Expression; Key -
Due before class on Sept. 16
Homework on Plasmids;
Key - Due before class on Sept. 23
Homework
on Regulation of Expression; Key -
Due before class on Sept. 25
Old Exams
(may not necessarily match style for this year)
2009 - Key
2008 - Exam - Key
2007 - Exam - Key
2006 - Exam -
Key
2005 - Exam
-
Key
2004 - Exam -
Key
2003 - Exam -
Key
2002 - Exam
2001 - Exam -
Key
2000 - Exam - Key
1999 - Exam - Key
Expression-secretion
DNA replication
Phage
Phage Display
scFv Phage Display
Regulation 1
Regulation 2
