Name
 

1. What would happen to the plasmid copy number of the F plasmid and ColE1 plasmid if you added the following antibiotics to an E. coli culture (the strain is sensitive to the antibiotics, but the question is not what happens if the culture dies or stops growing - it's how does the antibiotic affect the plasmid). In a sentence explain your answer. Hint - at one time one of these treatments was used to aid in plasmid extraction.

A. ColE1 - Rifampin

B. F plasmid - Rifampin

C. ColE1 - Chloramphenicol

D. F plasmid - Chloramphenicol

2. For the F plasmid describe what would happen to copy number of the plasmids if the binding site for RepE at the promoter for repE and [not the ori] was mutated to possess lower affinity for the protein.

3. Which of the following loci are necessarily cis-active? (Check all boxes that are correct.)
copA/incC of F
RNA-II of ColE1 
rop of ColE1 
oriV of F

4. What factors would determine the host range of an RNA regulated vs. protein regulated plasmid?

5.  So called allelic exchange suicide plasmids are based on lack of an essential protein for replication on the plasmid.  How can you construct a suicide plasmid using the RNA regulated model?